Infection:Article Title: Muscleblind-like 1 ( Mbnl1 ) regulates pre-mRNA alternative splicing during terminal erythropoiesis
Article Snippet: .. After spin infection, the virus solution was substituted by erythroid maintenance medium (StemSpan-SFEM; Stem Cell Technologies) with added recombinant mouse stem cell factor (100 ng/mL stem cell factor; R&D), recombinant mouse insulin-like growth factor-1 (40 ng/mL; R&D), and dexamethasone (100 nM; Sigma) and cultured overnight for recovery and expression of foreign genes. .. 27 The next morning, the green fluorescent protein (GFP) + cells (or the GFP and DsRed double-positive cells for rescue experiments) were sorted and cultured in Epo-only erythroid differentiation medium (Iscove modified Dulbecco's medium containing 15% FBS [Stemcell], 1% detoxified bovine serum albumin [Stemcell], 500 μg/mL holo-transferrin [Sigma-Aldrich], 0.5 U/mL Epo [Amgen], 10 μg/mL recombinant human insulin [Sigma-Aldrich], 2 mM l -glutamine [Invitrogen], and 1× Pen Strep [Invitrogen]) for 2 or 3 days before analysis.
Article Title: Enhanced phosphocholine metabolism is essential for terminal erythropoiesis
Article Snippet: .. After this spin infection, the virus supernatant was replaced with erythroid maintenance medium (StemSpan-SFEM; Stemcell Technologies) supplemented with 100 ng/mL recombinant mouse stem cell factor (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma-Aldrich), and 2 U/mL erythropoietin (Amgen), cultured at 37°C. .. Green fluorescent protein–positive (GFP + ) cells were sorted by flow cytometry after 16 hours and cultured for another 48 hours in erythroid differentiation medium (Iscove modified Dulbecco medium containing 15% [vol/vol] FBS; Stemcell Technologies), 1% detoxified bovine serum albumin (Stemcell Technologies), 500 μg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL epoetin (Amgen), 10 μg/mL recombinant human insulin (Sigma-Aldrich), and 2 mM l -glutamine (Invitrogen) at 37°C.
Virus:Article Title: Muscleblind-like 1 ( Mbnl1 ) regulates pre-mRNA alternative splicing during terminal erythropoiesis
Article Snippet: .. After spin infection, the virus solution was substituted by erythroid maintenance medium (StemSpan-SFEM; Stem Cell Technologies) with added recombinant mouse stem cell factor (100 ng/mL stem cell factor; R&D), recombinant mouse insulin-like growth factor-1 (40 ng/mL; R&D), and dexamethasone (100 nM; Sigma) and cultured overnight for recovery and expression of foreign genes. .. 27 The next morning, the green fluorescent protein (GFP) + cells (or the GFP and DsRed double-positive cells for rescue experiments) were sorted and cultured in Epo-only erythroid differentiation medium (Iscove modified Dulbecco's medium containing 15% FBS [Stemcell], 1% detoxified bovine serum albumin [Stemcell], 500 μg/mL holo-transferrin [Sigma-Aldrich], 0.5 U/mL Epo [Amgen], 10 μg/mL recombinant human insulin [Sigma-Aldrich], 2 mM l -glutamine [Invitrogen], and 1× Pen Strep [Invitrogen]) for 2 or 3 days before analysis.
Article Title: Enhanced phosphocholine metabolism is essential for terminal erythropoiesis
Article Snippet: .. After this spin infection, the virus supernatant was replaced with erythroid maintenance medium (StemSpan-SFEM; Stemcell Technologies) supplemented with 100 ng/mL recombinant mouse stem cell factor (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma-Aldrich), and 2 U/mL erythropoietin (Amgen), cultured at 37°C. .. Green fluorescent protein–positive (GFP + ) cells were sorted by flow cytometry after 16 hours and cultured for another 48 hours in erythroid differentiation medium (Iscove modified Dulbecco medium containing 15% [vol/vol] FBS; Stemcell Technologies), 1% detoxified bovine serum albumin (Stemcell Technologies), 500 μg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL epoetin (Amgen), 10 μg/mL recombinant human insulin (Sigma-Aldrich), and 2 mM l -glutamine (Invitrogen) at 37°C.
Article Title: In vitro production of red blood cells with sortaggable proteins
Article Snippet: .. Immediately following spin-infection, the virus-containing supernatant was aspirated and replaced with erythroid maintenance medium (StemSpan-SFEM (StemCell Technologies) supplemented with recombinant mouse stem cell factor (100 ng/ml SCF, R&D), recombinant mouse IGF-1 (40 ng/ml, R&D), dexamethasone (100 nM, Sigma), and erythropoietin (2 u/ml, Amgen)). ..
Article Title: Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin
Article Snippet: .. After this spin-infection, the virus supernatant was replaced with erythroid maintenance medium (StemSpan-SFEM; StemCell Technologies) supplemented with 100 ng/ml recombinant mouse SCF (R&D Systems), 40 ng/ml recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma), and 2 U/ml erythropoietin (Amgen) and cultured at 37 °C. .. GFP+ cells were sorted by flow cytometry after 16 h and cultured for another 48 h in erythroid differentiation medium (IMDM containing 15% (vol/vol) FBS (Stemcell), 1% detoxified bovine serum albumin (BSA; Stemcell), 500 μg/ml holo-transferrin (Sigma-Aldrich), 0.5 U/ml Epoetin (Epo; Amgen), 10 μg/ml recombinant human insulin (Sigma-Aldrich), and 2 mM L-glutamine (Invitrogen)) at 37 °C.
Article Title: In vitro production of red blood cells with proteins comprising sortase recognition motifs
Article Snippet: .. Immediately following spin-infection, the virus-containing supernatant was aspirated and replaced with erythroid maintenance medium (StemSpan-SFEM (StemCell Technologies) supplemented with recombinant mouse stem cell factor (100 ng/ml SCF, R&D), recombinant mouse IGF-1 (40 ng/ml, R&D), dexamethasone (100 nM, Sigma), and erythropoietin (2 u/ml, Amgen)). ..
Recombinant:Article Title: Muscleblind-like 1 ( Mbnl1 ) regulates pre-mRNA alternative splicing during terminal erythropoiesis
Article Snippet: .. After spin infection, the virus solution was substituted by erythroid maintenance medium (StemSpan-SFEM; Stem Cell Technologies) with added recombinant mouse stem cell factor (100 ng/mL stem cell factor; R&D), recombinant mouse insulin-like growth factor-1 (40 ng/mL; R&D), and dexamethasone (100 nM; Sigma) and cultured overnight for recovery and expression of foreign genes. .. 27 The next morning, the green fluorescent protein (GFP) + cells (or the GFP and DsRed double-positive cells for rescue experiments) were sorted and cultured in Epo-only erythroid differentiation medium (Iscove modified Dulbecco's medium containing 15% FBS [Stemcell], 1% detoxified bovine serum albumin [Stemcell], 500 μg/mL holo-transferrin [Sigma-Aldrich], 0.5 U/mL Epo [Amgen], 10 μg/mL recombinant human insulin [Sigma-Aldrich], 2 mM l -glutamine [Invitrogen], and 1× Pen Strep [Invitrogen]) for 2 or 3 days before analysis.
Article Title: Enhanced phosphocholine metabolism is essential for terminal erythropoiesis
Article Snippet: .. After this spin infection, the virus supernatant was replaced with erythroid maintenance medium (StemSpan-SFEM; Stemcell Technologies) supplemented with 100 ng/mL recombinant mouse stem cell factor (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma-Aldrich), and 2 U/mL erythropoietin (Amgen), cultured at 37°C. .. Green fluorescent protein–positive (GFP + ) cells were sorted by flow cytometry after 16 hours and cultured for another 48 hours in erythroid differentiation medium (Iscove modified Dulbecco medium containing 15% [vol/vol] FBS; Stemcell Technologies), 1% detoxified bovine serum albumin (Stemcell Technologies), 500 μg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL epoetin (Amgen), 10 μg/mL recombinant human insulin (Sigma-Aldrich), and 2 mM l -glutamine (Invitrogen) at 37°C.
Article Title: In vitro production of red blood cells with sortaggable proteins
Article Snippet: .. Immediately following spin-infection, the virus-containing supernatant was aspirated and replaced with erythroid maintenance medium (StemSpan-SFEM (StemCell Technologies) supplemented with recombinant mouse stem cell factor (100 ng/ml SCF, R&D), recombinant mouse IGF-1 (40 ng/ml, R&D), dexamethasone (100 nM, Sigma), and erythropoietin (2 u/ml, Amgen)). ..
Article Title: Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin
Article Snippet: .. After this spin-infection, the virus supernatant was replaced with erythroid maintenance medium (StemSpan-SFEM; StemCell Technologies) supplemented with 100 ng/ml recombinant mouse SCF (R&D Systems), 40 ng/ml recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma), and 2 U/ml erythropoietin (Amgen) and cultured at 37 °C. .. GFP+ cells were sorted by flow cytometry after 16 h and cultured for another 48 h in erythroid differentiation medium (IMDM containing 15% (vol/vol) FBS (Stemcell), 1% detoxified bovine serum albumin (BSA; Stemcell), 500 μg/ml holo-transferrin (Sigma-Aldrich), 0.5 U/ml Epoetin (Epo; Amgen), 10 μg/ml recombinant human insulin (Sigma-Aldrich), and 2 mM L-glutamine (Invitrogen)) at 37 °C.
Article Title: In vitro production of red blood cells with proteins comprising sortase recognition motifs
Article Snippet: .. Immediately following spin-infection, the virus-containing supernatant was aspirated and replaced with erythroid maintenance medium (StemSpan-SFEM (StemCell Technologies) supplemented with recombinant mouse stem cell factor (100 ng/ml SCF, R&D), recombinant mouse IGF-1 (40 ng/ml, R&D), dexamethasone (100 nM, Sigma), and erythropoietin (2 u/ml, Amgen)). ..
Cell Culture:Article Title: Muscleblind-like 1 ( Mbnl1 ) regulates pre-mRNA alternative splicing during terminal erythropoiesis
Article Snippet: .. After spin infection, the virus solution was substituted by erythroid maintenance medium (StemSpan-SFEM; Stem Cell Technologies) with added recombinant mouse stem cell factor (100 ng/mL stem cell factor; R&D), recombinant mouse insulin-like growth factor-1 (40 ng/mL; R&D), and dexamethasone (100 nM; Sigma) and cultured overnight for recovery and expression of foreign genes. .. 27 The next morning, the green fluorescent protein (GFP) + cells (or the GFP and DsRed double-positive cells for rescue experiments) were sorted and cultured in Epo-only erythroid differentiation medium (Iscove modified Dulbecco's medium containing 15% FBS [Stemcell], 1% detoxified bovine serum albumin [Stemcell], 500 μg/mL holo-transferrin [Sigma-Aldrich], 0.5 U/mL Epo [Amgen], 10 μg/mL recombinant human insulin [Sigma-Aldrich], 2 mM l -glutamine [Invitrogen], and 1× Pen Strep [Invitrogen]) for 2 or 3 days before analysis.
Article Title: Enhanced phosphocholine metabolism is essential for terminal erythropoiesis
Article Snippet: .. After this spin infection, the virus supernatant was replaced with erythroid maintenance medium (StemSpan-SFEM; Stemcell Technologies) supplemented with 100 ng/mL recombinant mouse stem cell factor (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma-Aldrich), and 2 U/mL erythropoietin (Amgen), cultured at 37°C. .. Green fluorescent protein–positive (GFP + ) cells were sorted by flow cytometry after 16 hours and cultured for another 48 hours in erythroid differentiation medium (Iscove modified Dulbecco medium containing 15% [vol/vol] FBS; Stemcell Technologies), 1% detoxified bovine serum albumin (Stemcell Technologies), 500 μg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL epoetin (Amgen), 10 μg/mL recombinant human insulin (Sigma-Aldrich), and 2 mM l -glutamine (Invitrogen) at 37°C.
Article Title: Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin
Article Snippet: .. After this spin-infection, the virus supernatant was replaced with erythroid maintenance medium (StemSpan-SFEM; StemCell Technologies) supplemented with 100 ng/ml recombinant mouse SCF (R&D Systems), 40 ng/ml recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma), and 2 U/ml erythropoietin (Amgen) and cultured at 37 °C. .. GFP+ cells were sorted by flow cytometry after 16 h and cultured for another 48 h in erythroid differentiation medium (IMDM containing 15% (vol/vol) FBS (Stemcell), 1% detoxified bovine serum albumin (BSA; Stemcell), 500 μg/ml holo-transferrin (Sigma-Aldrich), 0.5 U/ml Epoetin (Epo; Amgen), 10 μg/ml recombinant human insulin (Sigma-Aldrich), and 2 mM L-glutamine (Invitrogen)) at 37 °C.
Expressing:Article Title: Muscleblind-like 1 ( Mbnl1 ) regulates pre-mRNA alternative splicing during terminal erythropoiesis
Article Snippet: .. After spin infection, the virus solution was substituted by erythroid maintenance medium (StemSpan-SFEM; Stem Cell Technologies) with added recombinant mouse stem cell factor (100 ng/mL stem cell factor; R&D), recombinant mouse insulin-like growth factor-1 (40 ng/mL; R&D), and dexamethasone (100 nM; Sigma) and cultured overnight for recovery and expression of foreign genes. .. 27 The next morning, the green fluorescent protein (GFP) + cells (or the GFP and DsRed double-positive cells for rescue experiments) were sorted and cultured in Epo-only erythroid differentiation medium (Iscove modified Dulbecco's medium containing 15% FBS [Stemcell], 1% detoxified bovine serum albumin [Stemcell], 500 μg/mL holo-transferrin [Sigma-Aldrich], 0.5 U/mL Epo [Amgen], 10 μg/mL recombinant human insulin [Sigma-Aldrich], 2 mM l -glutamine [Invitrogen], and 1× Pen Strep [Invitrogen]) for 2 or 3 days before analysis.
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